Thursday, February 27, 2020
The Law of Agency and the Power of Attorney Case Study
The Law of Agency and the Power of Attorney - Case Study Example It is evidently clear from the discussion that in the case of Francis Gagnon, Joan her daughter and Frank Gagnon the son, it is clear that Joan had the right and authority for the transfer of Shelburne property under her creation and control. First, once the power of Attorney has been signed, it holds and in the event of revocation, then the agent must be served with a copy of the signed revocation documents. In the case stated, it is evident that Francis Gagnonââ¬â¢s revocation was never told to Joan explicitly, and, therefore, the power of Attorney still holds. Secondly, according to the power of Attorney, any decision that an agent makes on behalf of the principle stands. Additionally, Frank Gagnon never incapacitated for any reason, to revoke the already signed power of Attorney. Based on such a perspective, it is sufficiently clear that the revocation of the power of attorney was unlawful. Since an agent should always act in place of the principle, the property had, therefore , be transferred to the trust, and, therefore, was the trustââ¬â¢s property.
Tuesday, February 11, 2020
Determination of Protein using Biuret Reagent Lab Report
Determination of Protein using Biuret Reagent - Lab Report Example The methods used are all optical methods. This means that the level of protein concentration depends on optical characteristics (turbidity or absorption). These optical properties are measured using a tool called colorimeter or spectrophotometer. Other useful methods are volumetric, gravimetric, among others (Slater, 1986, 12). After the reaction between the reagents and the protein substrate, the peptide bond turns purple or violet for positive results. We cannot conclude that there is the presence of protein by candid colorimetric methods. This is because proteins are colorless. However, absolute estimations can be made to analyze the protein in the solution (Slater, 1986, 12). A stable color is achieved. It is always advisable to take all reading within the first 10mins for the purpose of clarity. In many experiments, biuret can be scaled using smaller sized cuvette, which consumes less protein. Proteins with abnormal low or high percentage of amino acids and aromatic side groups always gives low or high readings, respectively (Robinson, and Hogden, 1940, 32) In the case of bovine serum, albumin will obviously develop a linear relationship between the amount of protein and absorbance over a range of 0.5 to 20mg protein. However, analysis show assay below 0.5 mg is not reliable. In other cases, the upper limit may increase depending with the reagents. A straight line graph is achieved after plotting the relationship between protein concentration and absorbance. This means that there is a tremendous increase in absorbance with an increase in protein concentration (Layne, 1957, 23). The reagent is made up of potassium hydroxide. It also has traces of hydrated copper (II) sulphate and potassium sodium titrate. The blue color appears as a result of presence of proteins, pink and blue may also arise in the presence of short chained polypeptides. A biuret reagent is not required in all protein tests. It is
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